Why We Are Moving Toward Non-Viral Gene Editing

 

For years, viral vectors (like Lentivirus) have been the gold standard for getting new genes into T-cells. However, they are expensive and time-consuming to produce. Our lab is now transitioning toward Non-Viral Transposon Systems, specifically the Sleeping Beauty platform.

Why the switch?

• Speed: We can go from genetic design to a functional cell in half the time.

• Safety: Reduced risk of insertional mutagenesis (unwanted genetic mutations).

• Cost: Non-viral methods are significantly more affordable, a crucial step in making cell therapy accessible to all patients, not just those at elite medical centers.

We recently presented our optimized protocol at the Global Cell & Gene Summit, demonstrating that our non-viral cells maintain the same potency as their viral counterparts.

[Download the Technical Protocol] | [Watch the Conference Presentation]